http://dap.cooper.edu/ 2026-04-15T12:44:56-04:00 http://dap.cooper.edu/ http://dap.cooper.edu/lib/images/favicon.ico text/html 2012-03-23T10:36:13-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:a_s_camaro&rev=1332513373&do=diff Here is the completed (up to page 31) Camaro in Solidworks: text/html 2012-03-02T14:05:25-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:a_s_gym_design&rev=1330715125&do=diff Main Ideas: 1.) Treadmill Basketball An exerciser presets the treadmill to one of the following modes: walk, jog, or run. Based on the distance traveled in a given interval of time the treadmill outputs power to a launching device. The ball will be launched appropriately to be successfully shot through the hoop if the distance traveled meets a specified goal. The treadmill basketball system could be used as a game if two exercisers engage at the same time on two separate systems. text/html 2012-03-02T14:00:42-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:a_s_laboratory_work&rev=1330714842&do=diff Here are some examples of techniques and procedures carried out in the laboratory: DNA Transformation: Streaking: text/html 2012-03-02T14:18:05-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:a_s_rhino&rev=1330715885&do=diff Rhino Assignments Rubber Ducky Here are some pictures of rubber ducks that others have designed: Here is the Rubber Ducky modeled in Rhino: Racecar Here are several different views of the Racecar modeled in Rhino: text/html 2012-03-02T14:03:05-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:a_s_sketches&rev=1330714985&do=diff This homework assignment consisted of eight pages of design sketches pertaining to my particular subgroup of the EID 103 spring course, the iGem competition. Goal: USE THE BACTERIA TO ATTEMPT TO PRODUCE DIFFERENT COLOR WRITING UTENSILS Goal: USE THE BACTERIA AS A MEANS TO MANUFACTURE COMIC BOOKS BY PROVIDING THE DESIRED LAYOUT AND COLOR DISTRIBUTION IN A POTENTIALLY COST EFFICIENT METHOD text/html 2012-05-07T22:14:25-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:anthony_simonetti&rev=1336443265&do=diff Anthony Simonetti About Me I am currently pursuing a degree in the Bachelor of Science of Engineering program, but ultimately plan to obtain an mechanical engineering program. As part of the iGem subgroup of the EID 103 spring, I have even working to expand upon the previous work of Cooper and Columbia students as well as to design, prototype, and test my group's electrophoresis chamber. After completing the design process, we determined that our chamber successfully carries out its operation… text/html 2012-05-08T00:48:19-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:assign1&rev=1336452499&do=diff Assignment 1 Sketches * This assignment asked for ideas of uses for our group projects. In the case of the iGEM group, this involved uses for the luminescent quantum dots. [Lovingly stitched together in ms paint] [This file almost crashed my laptop] text/html 2012-05-08T00:47:05-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:assign2&rev=1336452425&do=diff Assignment 2 Sketch * This assignment asked to create an exercise room, employing creative devices to achieve fitness. text/html 2012-05-08T00:49:59-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:assign3&rev=1336452599&do=diff Assignment 3 (Rhino intro) * This assignment asked to create a CO2 Toy Car and Rubber Duck (Ducky) in the Rhino CAD program. * Ducky [Duck.duck] * Ducky [Duck] * CO2 Toy Car [CO2 Car] text/html 2012-05-08T00:51:08-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:assign4&rev=1336452668&do=diff Camaro * We were asked to model the side of a Camero in Solidworks, following the directions of a free tutorial found online. text/html 2012-03-02T10:16:19-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:bact1&rev=1330701379&do=diff Bacteria Streaking text/html 2012-03-30T14:23:24-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:bed_lifter&rev=1333131804&do=diff The Bed Lifting Machine Here are some photographs of the bed-lifting device: Here is the overall view of the machine: Below shows the bed frame at its ground state, and later at its final state: Below are the views that show the gear mechanism: text/html 2012-03-30T14:04:17-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:bom&rev=1333130657&do=diff Bill of Materials Below is the bill of materials for our bed lifting machine: Back to iGEM text/html 2012-05-09T09:46:13-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:end_of_year_show&rev=1336571173&do=diff End of the Year Show Related Material-iGEM Competition Group Group Members Yuta Makita Eric Ramos Anthony Simonetti Stanley George Group Mentors Alison Acevedo Dionne Lutz Photographs Refined Introduction Statement Gel electrophoresis is a process by which proteins (DNA) are separated by charge and molecular size. This a standard procedure used very commonly in labs. Due to the unavailability and cost of conventional gel electrophoresis chambers, we opted to design our ow… text/html 2012-05-08T11:51:35-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:eric_ramos&rev=1336492295&do=diff I am currently studying mechanical engineering at The Cooper Union. This webpage is focused on my work for the iGEM team at Cooper, for the engineering design class EID 103 during the spring 2012 semester. The iGEM group aims at generating bacteria capable of producing quantum dots. In March of 2012, focus was given to designing a gel electrophoresis chamber for separating DNA that would be used in the lab. We were successful in designing and creating the prototype of this chamber in April, phot… text/html 2012-03-02T15:09:51-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:experiment_1_protocol&rev=1330718991&do=diff Protocol for the First Experiment 3/2/2012 Protocol * Obtain bacterial culture with pre-transformed bacteria (with gene that synthesizes CdS) * Prepare subculture of bacteria cells into 200 mL of LB broth, OD600 = .6 at 37C * Prepare 6 samples: no bacteria control, no IPTG control, 3hr CdCl2, 2hr CdCl2, 1hr CdCl2 and a 3hr CdCl2 with no Na2S * The below procedure is based off of the summer iGEM procedure * Induce culture with .5 mM IPTG for 4 hours * Take 10 mL of LB medium with ka… text/html 2012-03-28T01:03:42-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:experiment_2_protocol&rev=1332911022&do=diff Protocol for the Second Experiment 3/28/2012-4/9/2012 Protocol Goal: Grow large amounts of the pET28+CDS7 plasmid, grow up large amounts of the chlorophenicol biobrick backbone, restriction digest both and ligate the two together. * Streak both plasmids on a plate. grow O/N (Day 1) * Inoculate a few minicultures with colonies. grow O/N (Day 2) * Miniprep the minicultures (Day 3) * Verify plasmid using gel electrophoresis (Day 3) * Digest plasmid with restriction enzymes (Day 4) *… text/html 2012-02-14T15:33:11-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:first_group_sketches&rev=1329251591&do=diff Below are the group sketches for the first assignment: Back to iGEM text/html 2012-05-08T11:53:44-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:gelchamber&rev=1336492424&do=diff Solidworks Model of Gel Electrophoresis Chamber Here are the screenshots of the prototype for the gel electrophoresis chamber, designed with my partner Yuta Makita during March and April 2012. text/html 2012-03-23T10:58:58-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:hoq&rev=1332514738&do=diff House of Quality Diagram Below is the pdf file to our diagram: [House of Quality] Back to iGEM text/html 2012-05-08T11:34:21-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:lab_work&rev=1336491261&do=diff Day 1 * March 1, 2012 (Start time: 15:15) * Procedure: * Incubate mini culture of bacteria with transformed gene at 37C, 200rpm shaking overnight Day 2 * March 2, 2012 (Start time: 13:15) * Sample name as follows: * Sample 1: Bacteria, CdCl2, Na2S, IPTG, 3 hrs. * Sample 2: No bacteria, CdCl2, Na2S, IPTG, 3 hrs. * Sample 3: Bacteria, CdCl2, Na2S, IPTG, 2 hrs. * Sample 4: Bacteria, CdCl2, Na2S, IPTG, 1 hr. * Sample 5: Bacteria, CdCl2, IPTG, 3 hrs. * Sample 6: Bacteria, Na2S,… text/html 2012-04-09T02:03:04-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:lesson_documents&rev=1333951384&do=diff Documents from Lessons Below are the documents used during our lessons with the mentors: The basics for bacterial transformation. Basic molar calculations/buffer formulas. Tutorial on spreading bacteria on a petri dish. Explanation on restriction enzyme digest. Below are several pages explaining the DNA ligation techniques. [DNA Ligation Protocol and Diagram Explanation] Back to iGEM text/html 2012-02-28T20:21:22-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:msdsexp1&rev=1330478482&do=diff Material Safety Data Sheets Click the below image to enlarge: Back to Experiment Protocol text/html 2012-05-07T20:41:50-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:photographs&rev=1336437710&do=diff Photographs taken from Lab Work February, 2012 Wednesday, 15th DNA transformed bacteria growing in petri dish From left to right, example, Anthony, Stanley, Yuta Monday, 20th Samples of commericially used LED lights, photo courtesy Anthony. text/html 2012-02-15T01:45:06-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:proposed_timeline&rev=1329288306&do=diff Proposed Timeline Below is a tentative timeline for our group: Back to iGEM text/html 2012-03-02T13:55:06-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:simonrhino&rev=1330714506&do=diff Rhino Assignments Rubber Ducky Racecar text/html 2012-03-23T10:52:49-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:stanley_george&rev=1332514369&do=diff About Me:I am currently a freshman at the Cooper Union pursuing a degree in mechanical engineering. As part of this semester's EID 103 class, my team members and I are aiming to advance the potential of genetically engineered bacteria in industrial and nonindustrial applications. As of late, we have been brainstorming various ideas which we think would highlight the true potential of genetically modified bacteria. text/html 2012-05-07T22:05:07-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:start&rev=1336442707&do=diff Array LEDs, more formally known as light emitting diodes, have a wide range of uses from industrial to home applications. They have a longer lifespan of up to 200 times the lifespan of traditional incandescent lightbulbs (1). However, in the manufacturing of the central component of the LED, the semiconductor, factories dump an excessive amount of “acids, cyanide compounds, organic solvents, silicon tetrachloride...[and] arsine gas” that is dumped into surrounding waters (2). text/html 2012-04-13T03:07:09-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:stepper_motors&rev=1334300829&do=diff Stepper Motor Related Material Below is the link to a pdf that shows an explanation to all of the parts in an arduino, stepper motor system. [Arduino Explanation] Below is another link to a youtube video showing the stepper motor's motion. Stepper Motor Motion Video text/html 2012-03-01T21:36:18-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:testificate&rev=1330655778&do=diff Test Page *ASDF text/html 2012-05-07T20:54:34-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:timeline&rev=1336438474&do=diff Minutes January 2012 Monday, 30th * Came up with a weekly meeting schedule (Tuesday, 2-4 p.m.) * Discussed the fundamental biological concepts behind the quantum dots * Dionne and Allison discussed the background of the iGem competition * Also gave an overview of the project's direction text/html 2012-02-14T15:40:17-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:ymassign&rev=1329252017&do=diff Preliminary Sketches-the first assignment Back to Yuta Makita text/html 2012-02-15T02:03:58-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:ymassign2&rev=1329289438&do=diff Assignment 2 Below is a picture of my idea of an interesting physical fitness room. The idea is developed from several concepts of human nature: * One is that people have a natural inclination of going the opposite way * As seen in beginning portion of the circuit, the person doing the exercise begins by going against an escalator * In addition: the circuit is built so the runner can run against the conveyer belt * Unlike traditional treadmills, the runner is able to move in a forward… text/html 2012-04-26T17:51:26-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:ymassign3&rev=1335477086&do=diff Assignment Number 3 Below are my pictures of the duck and racecar made in rhino. Please click on image for enlarged view. Note the duck had his birthday today (hence his little birthday hat)! Back to Yuta Makita text/html 2012-04-26T17:51:09-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:ymassign4&rev=1335477069&do=diff Camero Assignment Below is a model of a race car. Below are 3 views of my camero assignment. Back to Yuta Makita text/html 2012-05-08T11:38:13-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:ymphotos&rev=1336491493&do=diff Photographs taken from Lab Work February, 2012 Wednesday, 15th DNA transformed bacteria growing in a petri dish. Below is my petri dish; the bacteria (with JM109 coding DNA) is growing in Agarose gel with ampicillin. This was practice with spreading. text/html 2012-05-08T09:42:09-04:00 http://dap.cooper.edu/doku.php?id=start:classes:principlesofdesign:igem:yuta_makita&rev=1336484529&do=diff Biography I am a freshman Mechanical Engineer ('15) studying at the Cooper Union for the Advancement of Science and Art. I am interested in research based projects and will hope to contribute to the iGEM project. Please feel free to browse through this webpage to view my works and accomplishments through my first year as an engineering student.