=====Protocol for the Second Experiment 3/28/2012-4/9/2012=====
====Protocol====
//Goal: Grow large amounts of the pET28+CDS7 plasmid, grow up large amounts of the chlorophenicol biobrick backbone, restriction digest both
and ligate the two together.// \\ 
   - Streak both plasmids on a plate. grow O/N (Day 1)
   - Inoculate a few minicultures with colonies. grow O/N (Day 2)
   - Miniprep the minicultures (Day 3)
   - Verify plasmid using gel electrophoresis (Day 3)
   - Digest plasmid with restriction enzymes (Day 4)
   - Gel purify restriction digests (Day 5)
   - Ligate the purified products (Day 5)
   - Transform bacteria with ligation mixture. grow o/n (Day 6)
   - Inoculate miniculture of any successful transformants. grow o/n (Day 7)
   - Make glycerol stock (Day 8)
   - Miniprep/gel electrophoresis to verify (Day 9)
   * //Courtesy Dionne//
   * //Note: o/n denotes overnight//


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