=====Protocol for the First Experiment 3/2/2012=====
====Protocol====
   - Obtain bacterial culture with pre-transformed bacteria (with gene that synthesizes CdS)
   - Prepare subculture of bacteria cells into 200 mL of LB broth, OD600 = .6 at 37C
   - Prepare 6 samples: no bacteria control, no IPTG control, 3hr CdCl2, 2hr CdCl2, 1hr CdCl2 and a 3hr CdCl2 with no Na2S
   - The below procedure is based off of the summer iGEM procedure
   - Induce culture with .5 mM IPTG for 4 hours
   - Take 10 mL of LB medium with kanamycin
   - Add CdCl2 (approximately .00183g) until the final concentration is 1 mM
   - Incubate the sample for 3 hours (check progress each hour)
   - Add freshly prepared Na2S 9H20 (approximately .024018g) to final concentration 1 mM
   - Incubate samples at room temperature with end-over-end rotation for 1.5 hours
   - Wash final sample with water 3 times, place resulting sample under UV-vis and fluorescence spectrometry
====Possible derivatives to experiment====
   - Set negative control of experiment
     * Repeat steps 1-9 without the initial bacteria
     * This procedure ensures that the bacteria is synthesizing CdS (quantum dot)
   - Adjust the times of incubation
     * Adjust the the total times of incubation (suggested by steps 6 and 8)
     * Adjust by plus/minus 1 or 2 hours
     * Adjustment to see if incubation time affects the productivity in synthesis of CdS
====Material Safety Data Sheet====
[[MSDSexp1|Material Safety Data Sheet]]
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Note: Protocol is based off experiment conducted by Mi et al.\\
Article source: Biosynthesis and characterization of CdS quantum dots in genetically engineered E. Coli from Journal of Biotechnology

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